English version of Takeuchi Lab.'s page is here.

Last Update: June 26, 2014

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【竹内からの一言】

 生体は,膨大な数の構成単位を的確に制御している大変美しい機能体です。認識,反応,輸送,制御などの機能が組み合わされて達成される生体機能は,生体内の分子間に起こるさまざまな相互作用により発現しています。すべての生体機能の発現の引き金は、特定の分子がその受け皿を見つけて相互作用するところから始まります。すなわち、特定分子を他の分子と見分ける「分子認識」が生体機能の根本といえます。この「分子認識」現象の発現には,特定分子が結合するのに都合のよい3次元空間を作り出すという場の効果が重要で,この反応場を人工的に作り出すことが出来れば、生体機能を人工的に再現する第一歩となります。このような生体機能を人工的に再現した材料を設計・合成し工学的に応用することが、私の研究の当面の目標となっています。 

 世の中には面白い手法があります。「分子の鋳型を取る」とか「分子の物理的化学的性質を記憶させる」技術、モレキュラーインプリンティングです。石膏のギブスや金型を想像してもわかりますが,ある形の物体を混入させて材料を固めて作り,後からそれを抜くと、抜いた後にはその形に合った空洞が出来ます。これを分子レベルで行う技術です。このモレキュラーインプリンティングの技術を用いて分子の鋳型を取ると,生体高分子のように結合に都合のよい三次元空間が人工材料の中にできて「分子認識」機能が発現すると同時に、アミノ酸を主成分にする生体材料より安定な材料を使ったり,生分解性をもたせたり,生体適合性をもたせたり、分子認識に伴う信号を出す機能を付与したり、触媒活性をもたせて人工酵素のようにしたりと,人工材料のもつあらゆる特徴もそのまま持ち込むことができ、生体材料を超える人工材料を作ることが可能かもしれません。そのような夢を追いながら,国内外の博士研究員、大学院進学時あるいは卒研配属時に竹内研究室を希望してくれた学生と一緒に、モレキュラーインプリンティングを中心としたプロジェクト研究「分子の集積による分子認識・反応場の設計と合成に関する研究」に励んでいます。学部・大学院教育を通じて学生の才能と個性をうまく開花させながら、社会に貢献できる人材を育てたいと考えています。

 

設備:NMR(300MHz),MALDI-TOF-MS,LC-MS/MS(ESI.・トリプル四重極),LC-MS(ESI・TOF),GC-MS(EI),走査型電子顕微鏡、走査型プローブ顕微鏡、スラブ型光導波路分光測定装置、表面プラズモン共鳴測定装置,等温滴定カロリメトリー装置,蛍光寿命測定装置,蛍光分光光度計,高速液体クロマトグラフィー,紫外・可視分光光度計(積分球付),FT-IR(透過・反射),電気化学測定装置、水晶振動子化学計測装置、ドラフトその他有機合成用設備

 

学部生のためのやさしい研究紹介


メンバー


 

研究概要

 生体の機能や機構を模倣あるいは人工的に再現することにより、人工酵素/抗体などの人工有機ポリマーを創製し、工学的に応用する研究をコアにしている。すなわち、これまで天然の酵素や抗体が活躍してきたさまざまな場面で、安定で半永久の寿命を持つ優れた”代替品”を開発することを目的として、モレキュラーインプリンティング法という手法を独自に発展させ、分子の形状や化学的性質を記憶したポリマーの合成法を開発し、人工的に酵素や抗体の機能を創出する研究を行っている。その応用研究として、全く新しい概念の知的情報材料(人工バイオチップ)を開発し、生命科学、情報科学、環境科学その他の分野での適用を検討し、さらに、生体成分や生物活性物質、環境負荷物質などをターゲットにした分離・分析法やバイオセンサーの開発などを行っている。


 

研究業績

原著論文

(1)モレキュラーインプリンティング法によるバイオミメティック材料の創製

(2)バイオセンサーに関する研究

(3)非放射性レセプターアッセイによる薬物の定量法に関する研究

(4)生体関連物質の計測(バイオセンサーを除く)に関する研究

(5)マリンバイオテクノロジーに関する研究

(6)生体関連光学異性体の分離・分析に関する研究

著書・総説

講演・学会発表

 

原著論文 

(1)モレキュラーインプリンティング法によるバイオミメティック材料の創製

Takeuchi, T., Mori, T., Kuwahara, A., Ohta, T., Oshita, A., Sunayama, H., Kitayama Y., Ooya, T. Conjugated protein mimics with molecularly imprinted reconstructible and transformable regions assembled using space-filling prosthetic groups, Angew. Chem. Int. Ed. 2014, in press. (DOI: 10.1002/anie.201406852).

Sasaki, S., Kitayama, Y., Ooya, T., Takeuchi, T. Molecularly Imprinted Protein Recognition Thin Films Constructed by Controlled/Living Radical Polymerization, J. Biosci. Bioeng. 2014, in press. (DOI: 10.1039/C4PY00350K)

Kamon, Y., Matsuura, R., Kitayama, Y., Ooya, T., Takeuchi, T.. Precisely controlled molecular imprinting of glutathione-s-transferase by orientated template immobilization using specific interaction with an anchored ligand on a gold substrate, Polym. Chem. 2014, 5, 4764-4771. DOI: 10.1039/C4PY00350K.

Kitayama, Y., Takeuchi, T.. Localized surface plasmon resonance nanosensing of C-reactive protein with poly(2-methacryloyloxyethyl phosphorylcholine)-grafted-gold nanoparticles prepared by surface-initiated atom transfer radical polymerization, Anal. Chem. 2014, 86, 5587-5594. (DOI: 10.1021/ac501322x)

Sunayama , H., Ooya, T., Takeuchi, T. Fluorescent protein-imprinted polymers capable of signal transduction of specific binding events prepared by a site-directed two-step postimprinting modification, Chem. Commun. 2014, 50, 1347-1349. (DOI: 10.1039/c3cc47759b).

Inoue, N., Ooya, T., Takeuchi, T. Hydrophilic Molecularly Imprinted Polymers for Bisphenol A Prepared in Aqueous Solution, Microchim. Acta 2013, 180, 15, 1387-1392. (DOI: 10.1007/s00604-013-0996-5)

Suga, Y., Sunayama , H., Ooya, T., Takeuchi, T. Molecularly Imprinted Polymers Prepared Using Protein-Conjugated Cleavable Monomers Followed by Site-Specific Post-Imprinting Introduction of Fluorescent Reporter Molecules, Chem. Commun. 2013, 49, 8450-8452.表紙になりました

Inoue, Y., Kuwahara, A., Ohmori, K., Sunayama, H., Ooya, T., Takeuchi, T. Fluorescent Molecularly Imprinted Polymer Thin Films for Specific Protein Detection Prepared with Dansyl Ethylenediamine-Conjugated O-Acryloyl L-Hydroxyproline Biosens. Bioelectron. 2013, 48, 113-119.

Taguchi, Y., Takano, E., Takeuchi, T. SPR sensing of bisphenol A using molecularly imprinted nanoparticles immobilized on slab optical waveguide with consecutive parallel Au and Ag deposition bands coexistent with Bisphenol A-immobilized Au nanoparticles, Langmuir 2012, 28, 7083-7088.

Takano, E., Tanaka, F., Ooya, T., Takeuchi, T.Molecularly Imprinted Microspheres for Bisphenol A Prepared Using a Microfluidic Device, Anal. Sci. 2012, 28, 457-462.

Takano, E., Taguchi, Y., Ooya, T., Takeuchi, T. Dummy template-imprinted polymers for Bisphenol A prepared using a Schiff base-type template molecule with post-imprinting oxidation, Anal. Lett. 2012, 45, 1204-1213.

Inoue, J., Ooya, T., Takeuchi, T. Protein imprinted TiO2-coated quantum dots for fluorescent protein sensing prepared by liquid phase deposition, Soft Matter 2011, 7, 9681-9684.

Sunayama, H., Ooya, T., Takeuchi, T., Fluorescent Protein Recognition Polymer Thin Films Capable of Selective Signal Transduction of Target Binding Events Prepared by Molecular Imprinting with a Post-Imprinting Treatment, Biosens. Bioelectron. 2010, 26, 458.462.

Takeda, K., Kuwahara, A., Ohmori K., Takeuchi, T. Molecularly Imprinted Tunable Binding Sites Based on Conjugated Prosthetic Groups and Ion-Paired Cofactors, J. Am. Chem. Soc. 2009, 131, 8833-8838.

Shinmori, H., Furukawa, H., Fujimoto, K., Shimizu, H., Inouye, M., Takeuchi, T. Characteristic Fluorescence Behavior of Dialkynylpyrene Derivatives in Hydrophobic Cavity of Protein, Chem. Lett. 2009, 38, 84-85.

Murakami, S., Yamamoto, K., Shinmori H., Takeuchi, T. A Molecularly Imprinted Polymer for the Reconstruction of a Molecular Recognition Region, Chem. Lett. 2008, 37, 1028-1029.

Tatemichi, M., Sakamoto, M., Mizuhata, M., Deki, S., Takeuchi, T., Protein-templated Organic/inorganic Hybrid Materials Prepared by Liquid-phase Deposition, J. Am. Chem. Soc. 2007, 129, 10906 -10910.

Matsunaga, T., Hishiya, T., Takeuchi, T., Optimization of Functional Monomer Content in Protein-Imprinted Polymers, Anal. Lett. 2007, 40, 2633-2640.

Takeuchi, T., Akeda, K., Murakami, S., Shinmori, H., Inoue, S., Lee, W.-S., Hishiya, T., Photoresponsive Porphyrin-imprinted Polymers Prepared Using a Novel Functional Monomer Having Diaminopyridine and Azobenzene Moieties, Org. Biomol. Chem. 2007, 5, 2368-.2374.

Kutsumizu, R., Shinmori, H., Takeuchi, T., L-Lysine-linked Anthracenophane Derived from Thermodynamically Controlled Intermediates, Tetrahedron Lett. 2007, 48, 3225-3228.

Matsunaga, T., Hishiya, T., Takeuchi, T., Surface Plasmon Resonance Sensor for Lysozyme Based on Molecularly Imprinted Thin Films, Anal. Chim. Acta 2007, 591, 63-67.

Takeuchi, T., Goto, D., Shinmori, H., Protein Profiling by Protein Imprinted Polymer Array, Analyst 2007, 132, 101-103.(表紙になりました

Yane, T., Shinmori, H., Takeuchi, T., Atrazine Transforming Polymer Prepared by Molecular Imprinting with Post-imprinting Process, Org. Biomol. Chem., 2006, 4, 4469-4473.

Matsunaga, T., Takeuchi, T. Crystallized Protein-imprinted Polymer Chips, Chem. Lett. 2006, 35, 1030-1031.

Kubo, A., Shinmori, H., Takeuchi, T. Atrazine-imprinted Microspheres Prepared Using a Microfluidic Device, Chem. Lett, 2006, 588-589.

Takeuchi, T., Murase, N., Maki, H., Mukawa, T., Shinmori, H. Dopamine Selective Molecularly Imprinted Polymers via Post-imprinting Modification, Org. Biomol. Chem. 2006, 4, 565-568. (表紙になりました

Takeuchi, T., Minato, Y., Takase, M., Shinmori, H. Molecularly Imprinted Polymers with Halogen Bonding-based Molecular Recognition Sites, Tetrahedron Lett. 2005, 46, 9025-9027.

Lee, Woo-Sang, Takeuchi, T. Bisphenol A Analog-Imprinted Polymers Prepared by an Immobilized Template on a Modified Silica Microsphere Matrix, Anal. Sci. 2005, 21, 1125-1128.

Takeuchi, T., Mukawa, T., Shinmori, H. Signaling Molecularly Imprinted Polymers: Molecular Recognition-based Sensing Materials, Chem. Records 2005, 5, 263-275.

Navarro Villoslada, F., Takeuchi, T. Multivariate Analysis and Experimental Design In the Screening of Combinatorial Libraries of Molecular Imprinted Polymers, Bull. Chem. Soc. Jpn. 2005, 78, 1354-1361.

Kubo, H., Yoshioka, N., Takeuchi, T. Fluorescent Imprinted Polymers Prepared with 2-Acrylamidoquinoline as a Signaling Monomer, Org. Lett. 2005, 7, 359 - 362.

Takase, M., Nakajima, A., Takeuchi, T. Synthesis of an Extended Hexagonal Molecule as a Highly Symmetrical Ligand, Tetrahedron Lett. 2005, 46, 1739-1742.

Takeuchi, T, Seko, A., Mukawa, T. Molecularly Imprinted Polymers with Signaling Function Based on the UV-Vis Spectral Change by Diastereoselective Binding Events, Bull. Chem. Soc. Jpn. 2005, 78, 356-360.

Takeuchi, T., Ugata, S., Masuda, S., Matsui, J., Yane, T., Takase, M. Atrazine Tranformation Using Synthetic Enzymes Prepared by Molecular Imprinting, Org. Biomol. Chem. 2004, 2, 2563-2566.

Ikegami T., Lee, W.-S., Nariai, H., Takeuchi, T. Covalent Molecular Imprinting of Bisphenol A Using Its Diesters Followed by the Reductive Cleavage with LiAlH4, J. Chromatogr. B 2004, 804, 197-201.

Ikegami T., Lee, W.-S., Nariai, H., Takeuchi, T. Synthetic Polymers Adsorbing Bisphenol A and Its Analogues Prepared by Covalent Molecular Imprinting Using Bisphenol A Dimethacrylate as a Template Molecule, Anal. Bioanal. Chem. , 2004, 378, 1898-1902.

Ikegami, T., Mukawa, T., Nariai, H., Takeuchi, T. Bisphenol A-recognition Polymers Prepared by Covalent Molecular Imprinting, Anal. Chim. Acta 2004, 504, 131-135.

Kubo, H., Player, T. N., Shinoda, S Tsukube, H., Nariai, H., Takeuchi, T. Chiral Recognition of Octadentate Na+ Complex with Tetra-Armed Cyclen by Molecularly Imprinted Polymers, Anal. Chim. Acta 2004, 504, 137-140.

Kubo, H., Nariai, H. Takeuchi, T "Multiple Hydrogen Bonding-based Fluorescent Imprinted Polymers for Cyclobarbital Prepared with 2,6-Bis(acrylamido)pyridine", Chem. Commun.. 2003, 2792-2793.

Shoji, R., Takeuchi, T., Kubo, I. Atrazine Sensor Based on Molecularly Imprinted Polymer Modified Gold Electrode, Anal. Chem. 2003, 75, 4882-4886.

Mukawa, T., Goto, T., Nariai, H., Aoki, Y., Imamura, A., Takeuchi, T. Novel Strategy for Molecular Imprinting of Phenolic Compounds Utilizing Disulfide Templates, J. Pharm. Biomed.Anal. 2002,30, 1943-1947.

Mukawa, T., Goto, T., Takeuchi, T. Post-Oxidative Conversion of Thiol Residue to Sulfonic Acid in the Binding Sites of Molecularly Imprinted Polymers: Disulfide Based Covalent Molecular Imprinting for Basic Compounds, Analyst 2002, 127, 1407-1409.

Kugimiya, A., Takeuchi, T. Molecular Recognition in Indoleacetic Acid-Imprinted Polymers: Effect of 2-Hydroxyrthyl Methacrylate Contents, Anal. Bioanal. Chem. 2002, 372, 305-307.

Kugimiya, A., Kuwada, Y., Takeuchi, T. Preparation of Sterol-Imprinted Polymers with the Use of 2-(Methacryloyloxy)ethyl Phosphate, J. Chromatogr. A 2001, 938, 131-135.

Takeuchi, T., Mukawa, T., Matsui, J., Higashi, M., Shimizu, K. D. Molecularly Imprinted Polymers with Metalloporphyrin-Based Molecular Recognition Sites Coassembled with Methacrylic acid, Anal. Chem. 2001, 73, 3869-3874.

Takeuchi, T., Fukuma, D., Matsui, J., Mukawa, T. Combinatorial Molecular Imprinting for Formation of Atrazine Decomposing Polymers, Chem. Lett. 2001, 530-531.

Kugimiya, A., Takeuchi, T. Surface Plasmon Resonance Sensor Using Molecularly Imprinted Polymer for Detection of Sialic Acid, Biosens. Bioelectron. 2001, 16, 1059-1062.

Kugimiya, A., Mukawa, T., Takeuchi, T. Synthesis of 5-Fluorouracil-Imprinted Polymers with Multiple Hydrogen Bonding Interaction, Analyst 2001, 126, 772-774.

Takeuchi, T., Seko A., Matsui, J., Mukawa, T. Molecularly Imprinted Polymer Library on a Microtiter Plate: High-throughput Synthesis and Assessment of Cinchona Alkaloid-imprinted Polymers, Instrument. Sci. Technol. 2001, 29, 1-9.

Matsui, J., Higashi, M., Takeuchi, T. Molecularly Imprinted Polymer as 9-Ethyladenine Receptor Having a Porphyrin-based Recognition Center, J. Am. Chem. Soc. 2000, 122, 5218-5219.

Matsui, J., Kubo, H., Takeuchi, T. Molecularly Imprinted Fluorescent-shift Receptors Prepared with 2-(Trifluoromethyl)acrylic Acid, Anal. Chem. 2000, 72, 3286-3290.

Takeuchi, T., Dobashi, A., Kimura, K. Molecular Imprinting of Biotin Derivatives and Its Application to Competitive Binding Assay Using Non-isotopic Labeled Ligands, Anal. Chem. 2000, 72, 2418-2422.

Matsui, J., Fujiwara, K., Ugata, S., Takeuchi, T. Solid-Phase Extraction with a Dibutylmelamine-imprinted Polymer as Triazine Herbicide-selective Sorbent, J. Chromatogr. A, 2000, 889, 25-31.

Kugimiya, A., Yoneyama, H., Takeuchi, T. Sialic Acid Imprinted Polymer-Coated Quartz Crystal Microbalance, Electroanalysis 2000, 12, 1322-1326.

Matsui, J., Fujiwara, K., Takeuchi, T. Atrazine-Selective Polymers Prepared by Molecular Imprinting of Trialkylmelamines as Dummy Template Species of Atrazine, Anal. Chem. 2000, 72, 1810-1813.

Takeuchi, T., Matsui, J. Miniaturized Molecularly Imprinted Continuous Polymer Rods, J. High Resolution Chromatogr. 2000, 23, 44-46.

Takeuchi, T., Fukuma, D., Matsui, J. Combinatorial Molecular Imprinting: An Approach to Synthetic Polymer Receptors, Anal. Chem. 1999, 71, 285-290.

Kugimiya, A., Takeuchi, T. Molecularly Imprinted Polymer-Coated Quartz Crystal Microbalance for Detection of Biological Hormone, Electroanalysis 1999, 11, 1158-1160.

Kugimiya, A., Takeuchi, T. Effects of 2-Hydroxyethyl Methacrylate on Polymer Network and Interaction in Hydrophilic Molecularly Imprinted Polymers, Anal. Sci. 1999, 15, 29-33.

Kugimiya, A., Takeuchi, T. Application of Indoleacetic acid-Imprinted Polymer to Solid Phase Extraction, Anal. Chim. Acta 1999, 395, 251-255.

Matsui, J., Tachibana, Y., Takeuchi, T. Molecularly Imprinted Receptor having Metalloporphyrin-based Signaling Binding Site, Anal. Commun. 1998, 35, 225-227.

Matsui, J., Kubo, H., Takeuchi, T. Design and Preparation of Molecularly Imprinted Atrazine-Receptor Polymers: Investigation of Functional Monomers and Solvents. Anal. Sci. 1998, 14, 699-702.

Matsui, J., Nicholls, I. A., Takeuchi, T. Molecular Recognition in Cinchona Alkaloid Molecular Imprinted Polymer Rods. Anal. Chim. Acta 1998, 365, 89-93.

Kugimiya, A., Matsui, J., Abe, H., Aburatani, M., Takeuchi, T. Synthesis of Castasterone Selective Polymers Prepared by Molecular Imprinting. Anal. Chim. Acta 1998, 365, 75-79.

Takeuchi, T., Matsui, J. Recognition and Sensing of a Nucleobase by a Molecularly Imprinted Artificial Receptor, Nucleic Acids Symposium Series 1998, 39, 213-214.

Yano, K.,Tanabe, K., Takeuchi, T., Matsui, J., Ikebukuro, K., Karube, I. Molecularly Imprinted Polymers which Mimic Hydrogen Bonds between Nucleotide Bases, Anal. Chim. Acta 1998, 363, 111-117.

Yano, K, Nakagiri, T., Takeuchi, T., Matsui, J., Ikebukuro, K., Karube, I. Stereoselective Recognition of Dipeptide Derivatives in Molecularly Imprinted Polymers Which Incorporate an L-Valine Derivative as a Novel Functional Monomer, Anal. Chim. Acta 1997, 357, 91-98.

Matsui, J., Takeuchi, T. A Molecularly Imprinted Polymer Rod as Nicotine Selective Affinity Media Prepared with 2-(Trifluoromethyl)acrylic Acid, Anal. Commun. 1997, 34, 199-200.

Matsui, J., Doblhoff-Dier, O., Takeuchi, T. 2-(Trifluoromethyl)acrylic Acid: A Novel Functional Monomer in Non-covalent Molecular Imprinting, Anal. Chim. Acta. 1997, 343, 1-4.

Kugimiya, A., Matsui, J., Takeuchi, T. Sialic acid-imprinted polymers using noncovalent interactions, Materials Sci. Eng. 1997, C4, 263-266.

Matsui, J., Okada, M., Tsuruoka, M., Takeuchi, T. Solid-Phase Extraction of a Triazine Herbicide Using a Molecularly Imprinted Synthetic Receptor, Anal. Commun. 1997, 34, 85-87.

Matsui, J., Nicholls, I. A., Takeuchi, T. Highly Stereoselective Molecular Imprinted Polymer Synthetic Receptors for Cinchona Alkaloids, Tetrahedron: Asymm. 1996, 7, 1357-1361.

Matsui, J., Nicholls, I. A., Takeuchi, T., Mosbach, K., Karube, I. Metal Ion Mediated Recognition in Molecularly Imprinted Polymers, Anal. Chim. Acta 1996, 335, 71-77.

Matsui, J., Kaneko, A., Miyoshi, Y., Yokoyama, K., Tamiya, E., Takeuchi, T.
A Molecularly Imprinted Nicotine-selective Polymer, Anal. Lett. 1996, 29, 2071-2078.

Kugimiya, A., Takeuchi, T., Matsui, J., Yano, K., Karube, I. Recognition in Novel Molecularly imprinted Polymer Sialic Acid Receptors in Aqueou Media, Anal. Lett. 1996, 29, 1099-1107.

Piletsky, S. A., Piletskaya, E. V., Elgersma, A. V., Yano, K., Takeuchi, T., Karube, I., Panasyuk, T. I., El'skaya, A. V. A Biomimetic Receptor System for Sialic Acid Based on Molecular Imprinting, Anal. Lett. 1996, 29, 157-170.

Kato, T., Takeuchi, T., Karube, I. Bis-imidazolyl Cleft-shaped Mimic of the Active Site of Ribonuclease A, J. Chem. Soc., Chem. Commun. 1996, 953-954.

Matsui, J., Miyoshi, Y., Doblhoff-Dier, O., Takeuchi, T. A Molecularly Imprinted Synthetic Polymer Receptor Selective for Atrazine, Anal. Chem. 1995, 67, 4404-4408.

Matsui, J., Miyoshi, Y., Matsui, R., Takeuchi, T. Rod-type Affinity Media for Liquid Chromatography Prepared by In-situ-molecular imprinting, Anal. Sci. 1995, 11, 1017-1019.

Tanabe, K., Takeuchi, T., Matsui, J., Ikebukuro, K., Yano, K., Karube, I. Recognition of Barbiturates in Molecularly Imprinted Copolymers using Multiple Hydrogen Bonding, J. Chem. Soc. Chem.Commun. 1995, 2303-2304.

Matsui, J., Miyoshi, Y., Takeuchi, T. Fluoro-functionalized Molecularly Imprinted Polymers Selective for Herbicides, Chem. Lett. 1995, 1007-1008.

Matsui, J., Doblhoff-Dier, O., Takeuchi, T. Atrazine-selective Polymer Prepared by Molecular Imprinting Technique, Chem. Lett. 1995, 489.

Kugimiya, A., Matsui, J.,Takeuchi, T.,Yano, K., Muguruma, H.,Elgersma, A. V., Karube, I. Recognition of Sialic Acid Using Molecularly Imprinted Polymer, Anal. Lett. 1995, 28, 2317-2323.

Matsui, J., Kato, T. Takeuchi, T., Suzuki, M., Yokoyama, K., Tamiya, E., Karube, I. Molecular Recognition in Continuous Polymer Rods Prepared by a Molecular Imprinting Technique, Anal. Chem. 1993, 65, 2223-4.

研究業績のトップに戻る

(2)バイオセンサーに関する研究

Murata, A., Ooya, T., Takeuchi, T. Two-Layer Reflectometric Interference Spectroscopy-Based Immunosensing for C-Reactive Protein, Microchim. Acta 2014, in press. (DOI: 10.1007/s00604-014-1334-2)

Choi, H. W, Sakata, Y., Ooya, T., Takeuchi, T. Reflectometric Interference Spectroscopy-based Immunosensing Using Immobilized Antibody via His-tagged Recombinant Protein A J. Biosci. Bioeng. 2014, in press. (DOI: 10.1016/j.jbiosc.2014.06.017)

Murata, A., Ooya, T., Takeuchi, T. Simple immobilization of antibody in organic/inorganic hybrid thin films for immunosensing, Biosens. Bioelectron. 2013, 43, 45-49.

Kurihara,Y.,Takama, M., Masubuchi, M., Ooya, T., Takeuchi, T. Microfluidic Reflectometric Interference Spectroscopy-based Sensing for Exploration of Protein-Protein Interaction Conditions, Biosens. Bioelectron. 2013, 40, 247-251.

Kurihara,Y.,Takama, M., Sekiya, T., Yoshihara, Y., Ooya, T., Takeuchi, T. Fabrication of Carboxylated Silicon Nitride Sensor Chips for Detection of Antigen?Antibody Reaction Using Microfluidic Reflectometric Interference Spectroscopy, Langmuir 2012, 28, 13609-13615.

Choi, H. G., Kurihara, Y., Ooya, T., Takeuchi, T.Label-free Detection of C-reactive Protein Using Reflectometric Interference Spectroscopy-based Sensing System, Anal. Chim. Acta 2012, 728, 64-68.

Choi, H. G., Takahashi, H., Ooya, T., Takeuchi, T. Label-free Detection of Glycoproteins Using Reflectometric Interference Spectroscopy-Based Sensing System with Upright Episcopic Illumination, Anal. Methods 2011, 3, 1366-1370.

Ikebukuro, K., Wakamaru, K., Karube, I., Kubo, I., Inagawa, M., Sugawara, T., Arikawa, Y., Suzuki, M., Takeuchi, T., Phosphate Sensing System Using Pyruvate Oxidase and Chemiluminescence Detection, Biosens. Bioelectron. 1996, 11, 959-965.

Ikebukuro, K., Nishida, R., Yamamoto, H., Arikawa, Y., Nakamura, H., Suzuki, M., Kubo, I., Takeuchi, T., Karube, I. A novel biosensor system for the determination of phosphate,J. Biotechnol. 1996, 48, 67-72.

Mitsubayashi, M., Yokoyama, K., Takeuchi, T., Tamiya, E., Karube, I. Monitoring Sweat Lactate, Trans. Mat. Res. Soc. Jpn. 1994, 15A, 429-432.

Mitsubayashi, K., Dicks, J. M., Yokoyama, K., Takeuchi, T., Tamiya, E., Karube, I. A Flexible Biosensor for Glucose, Electroanalysis 1995, 7, 83-87.

Mitsubayashi, K., Yokoyama, K., Takeuchi, T., Tamiya, E., Karube, I. Monitoring of Sweat Lactate, Trans. Mater. Res. Soc. Jpn. 1994, 15A, 429-432.

Mitsubayashi, K., Ogasawara, K., Yokoyama, K., Takeuchi, T., Tsuru T., Karube, I. Measurement of Tear Electrolyte Concentration and Turnover Rate Using a Flexible Conductimetric Sensor, Technol. Health Care 1995, 3, 117-121.

Yokoyama, S., Yokoyama, K., Takeuchi, T., Tamiya, E., Karube, I. Urobilin Measurement Based on Peroxyoxalate Chemiluminescence, Trans. Mater. Res. Soc. Jpn. 1994, 15A, 433-436.

Kubo, I., Karube, I., Takeuchi, T., Furusawa, M., Arikawa, Y., Kanagawa, T. A Biosensor Based on Thiobacillus thioparus for Measuring Thiosulfate and Methanethiol, Can. J. Microbiol. 1995, 41, 366-371.

Imai, S., Suzuki, M., Takeuchi, T., Tamiya, E., Karube, I. Total Protein Sensor Based on Piezoelectric Quartz Crystal, Anal. Chim. Acta 1994, 292, 65-70.

Yokoyama, K., Sasaki, S., Ikebukuro, K., Takeuchi, T., Karube, I., Tokitsu, Y., Masuda, Y. Biosensing Based on NADH Detection Coupled to Electrogenerated Chemiluminescence from Ruthenium Tris (2,2'-Bipyridine), Talanta 1994, 41, 1035-1040.

Nomura, Y., Ikebukuro, K., Yokoyama, K., Takeuchi, T., Arikawa, Y., Ohno, S., Karube, I. A Novel Microbial Sensor for Anionic Surfactant Determination, Anal. Lett. 1994, 27, 3095-3108.

Murakami, Y., Uchida, T., Takeuchi, T., Tamiya, E., Karube, I., Suda, M. Micromachined Electrochemical Flow Cell for Biosensing, Electroanalysis 1994, 6, 735-739.

Murakami, Y., Suda, M., Yokoyama, K., Takeuchi, T., Tamiya, E., Karube, I. Micromachined Enzyme Reactor for FIA System, Microchem. J. 1994, 49, 319-325.

Mitsubayashi, K., Yokoyama, K., Takeuchi, T., Karube, I. Gas-Phase Biosensor for Ethanol, Anal. Chem. 1994, 66, 3297-3302.

Mitsubayashi, K., Yokoyama, K., Takeuchi, T., Tamiya, E., Karube, I. Flexible Conductimetric Sensor, Anal. Chem. 1993, 65, 3586-3590.

Murakami, Y., Takeuchi, T., Yokoyama, K., Tamiya, E., Karube, I, Suda, M. Integration of Enzyme-immobilized Column and Electrochemical Flow Cell Using Micromachining Techniques for the Glucose Detection System, Anal. Chem. 1993, 65, 2731-2735.

Tamiya, E., Sugiura, Y., Akiyama, A., Suzuki, M., Takeuchi, T. and Karube, I. Ultra-Micro Glutamate Sensor Using Platinized Carbon Fiber Electrode Integrated Counter Electrode, Sensors and Actuators 1993, B10, 179-184.

Navera, E. N., Suzuki, M., Takeuchi, T., Tamiya, E., Karube, I. Nafion-Coated Carbon Fiber for Acetylcholine and Choline Sensors, Electroanalysis 1993, 5, 17-22.

Navera, E. N., Yamashita, J., Suzuki, M., Yokoyama, K., Tamiya, E., Takeuchi, T., Karube, I. Micro-Choline Sensor for Acetylcholinesterase Determination, Anal.Chim. Acta 1993, 281, 673-679.

Hyun, C. K., Tamiya, E., Takeuchi, T., Karube, I. A Novel BOD Sensor Based on Bacterial Luminescence, Biotechnol. Bioeng. 1993, 41, 1107-1111.

Yokoyama, K., Nakajima, K., Uchiyama, S., Suzuki, S., Suzuki, M., Takeuchi, T., Tamiya, E., Karube, I Mediated Micro Glucose Sensors Using 2 mm Platinum Electrode, Electroanalysis 1992, 4, 859-864.
      
Iwasaki, Y.,Suzuki, M., Takeuchi, T., Tamiya, E., Karube, I., Nishiyama, M., Horinouchi, S., Beppu, T., Kadoi, H., Uchiyama, S., Suzuki, S. Direct Electron Transfer Reaction of a Blue Protein from Alcaligenes faecalis Strain-6, Electroanalysis 1992, 4, 756-770.

Iwasaki, Y., Takeuchi, T., Tamiya, E., Karube, I., Nishiyama, M., Horinouchi, S., Beppu, T., Kadoi, H., Uchiyama, S., Suzuki, S., Suzuki, M. Electrocatalysis of Nitrate Reductase from Alcaligenes faecalis S-6 Mediated by Native Redox Partner, Electroanalysis 1992, 4, 771-776.

研究業績のトップに戻る

(3)非放射性レセプターアッセイによる薬物の定量法に関する研究

Takeuchi, T., Nishikawa, T., Matsukawa, R., Matsui, J. Non-isotopic Receptor Assay for Benzodiazepine drugs Using Time-resolved Fluorometry, Anal. Chem. 1995, 67, 2655-2658.

Takeuchi, T., Yoshida, M., Kabasawa, Y., Matsukawa, R., Tamiya, E., Karube, I. Time-resolved Fluorometric Receptor Assay for Benzodiazepines, Anal. Lett. 1993, 26, 1535-1545.

Takeuchi, T., Tanaka, S., Rechnitz, G. A. Biotinylated-1012S Conjugate as a Probe Ligand for Benzodiazepine Receptors: Characterization of Receptor Binding Site and Receptor Assay for Benzodiazepine Drugs, Anal. Biochem. 1992, 203, 158-162.

Chen, L., Takeuchi, T., Rechnitz, G. A. Development of a Nonisotopic Acetylcholine Receptor Assay for the Investigation of Cholinergic Ligands, Anal. Chim. Acta 1992, 267, 55-62.

Tanaka, S., Takeuchi, T., Rechnitz, G. A. Non-Isotopic Receptor Assay for Benzodiazepines Using a Biotin-labeled Ligand and Biotin Immobilized Microtiter Plate, J. Chromatogr. 1992, 597, 443-448.

Takeuchi, T., Tanaka, S., Rechnitz, G. A. Non-Isotopic Receptor Assay for Benzodiazepine Drugs Using Biotin-Benzodiazepine Conjugate, Anal. Lett. 1991, 24, 2111-2121.

Takeuchi, T., Rechnitz, G. A. Nonisotopic Receptor-Binding Assay for Benzodiazepine Receptors Utilizing a Fluorophore Labeled Ligand, Anal. Biochem. 1991, 194, 250-255.

Takeuchi, T., Tham,S. Y., Rechnitz, G. A. Biotin Binding Assay Utilizing Avidin- Peroxidase Conjugate and Immobilized Iminobiotin on Polystyrene Beads, Anal. Chim. Acta 1991, 251, 291-295.

Takeuchi, T. and Rechnitz, G. A. Enzymatic Solid-Phase Assay for Biotin and a Biotin- Benzodiazepine Conjugate, Bioconjugate Chem. 1990, 1, 227-230.

研究業績のトップに戻る


(4)生体関連物質の計測(バイオセンサーを除く)に関する研究

Song, M. I., Iwata, K., Yamada, M., Yokoyama, K., Takeuchi, T., Tamiya, E., Karube, I.
Multisamples Analysis Using an Array of Microreactors for an Alternating-Current Field-Enhanced Latex Immunoassay, Anal. Chem. 1994, 66, 778-781.

Sekine, Y., Suzuki, M., Takeuchi, T., Tamiya, E. and Karube, I. Highly Sensitive Methanol Determination System Based on Chemiluminescence Using Flow Injection Analysis, Anal. Chim. Acta 1993, 280, 179-184.

Takeuchi, T., Kabasawa, Y., Horikawa, R. and Tanimura, T.
Mechanized Assay of Serum Cholinesterase Activity by Specific Colorimetric Detection of Released Acid, Clin. Chim. Acta 1992, 205, 117-126.
Takeuchi, T., Kabasawa, Y. and Tanimura, T. Continuous Separation of Lanthanides by Countercurrent Fractional Extraction, J. Chromatogr. 1991, 538, 125-131.

Takeuchi, T., Kabasawa, Y., Horikawa, R. and Tanimura, T. Flow Injection Determination of Drugs by Specific Detection of Carboxylic Acids, Analyst 1988, 113, 1673-1675.

Takeuchi, T., Kabasawa, Y., Horikawa, R. and Tanimura, T. Automated Assay of Serum Cholinesterase Activity, J. Pharm. Sci., 1987, 76, S4.

Takeuchi, T., Horikawa, R. and Tanimura, T. Spectrophotometric Determination of Carboxylic Acids by Ferric Hydroxamate Formation with Water-Soluble Carbodiimide, Anal. Lett. 1980, 13 (A7), 603-609.

研究業績のトップに戻る


(5)マリンバイオテクノロジーに関する研究

Matsukawa, R., Dubinsky, Z., Masaki, K., Takeuchi, T., Karube, I. Enzymatic Screening of Macroalgae as a Potential Source of Natural Antioxidants, Appl. Biochem. Biotechnol. 1997, 66, 239-247.

Matsukawa, R., Dubinsky, Z., Kishimoto, E., Masaki, K., Masuda, Y., Takeuchi, T., Chihara, M., Yamamoto, Y., Niki, E., Karube, I. A Comparison of Screening Methods for Antioxidant Activity in Seaweeds, J. Appl. Phycol. 1997, 9, 29-35.

Lee, T.-Y., Gotoh, N., Niki, E., Yokoyama, K., Tsuzuki, M., Takeuchi, T., Karube, I. Chemiluminescence Detection of Red Tide Phytoplankton Chattonella marina, Anal. Chem. 1995, 67, 225-228.

Lee, T.-Y., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. Quantitative Determination of Cyanobacteria in Mixed Phytoplankton Assemblages by an in vivo Fluorimetric Method, Anal. Chim. Acta 1995, 302, 81-87.

Lee, T.-Y., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. In vivo Fluorometric Method for Early Detection of Cyanobacterial Waterblooms, J. Appl. Phycol. 1994, 6, 489-495.

Takeuchi, T., Yokoyama, K., Imai, O., Masuda, Y., Utsunomiya, K., Kobayashi, K., Suzuki, M., Tamiya, E., Karube, I. Photosynthetic Activity Sensor Based on Oxygen Electrode Integrated with Optical Fibers, Anal. Chim. Acta 1993, 276, 65-68.

Hanagata, N., Ito, A., Uehara, H., Asari, F., Takeuchi, T., Karube, I. Behavior of Cell Aggregate of Carthamus tinctorius L. Cultured Cell and Correlation with Red Pigment Formation, J. Biotechnol. 1993, 30, 259-269.

Hanagata, N., Uehara, H., Ito, A., Takeuchi, T., Karube, I. Elicitor for Red Pigment Formation in Carthamus tinctorius Cultured Cells, J. Biotechnol. 1994, 34, 71-77.

Takeuchi, T., Utsunomiya, K., Kobayashi, K., Owada, M., Karube, I. Carbon Dioxide Fixation by a Unicellular Green Alga Oocystis sp., J. Biotechnol. 1992, 205, 117-126.

Hanagata, N., Takeuchi, T., Fukuju, Y, Barnes, D. J., Karube, I. Tolerance of Microalgae to High CO2 and High Temperature, Phytochemistry 1992, 31, 3345-3348.

研究業績のトップに戻る


(6)生体関連光学異性体の分離・分析に関する研究

Takeuchi, T., Yamazaki, S., Tanimura, T. Enantioselective Solubilization of DL-Amino Acids in Organic Solvents Containing N-n-Alkyl-L-Proline and Copper(II) Ions, Anal. Chim. Acta 1991, 242, 291-294.

Yamazaki, S., Takeuchi, T., Tanimura, T. Direct Enantiomeric Separation of b-Amino Acids and b-Amino Alcohols by Ligand-Exchange Chromatography, J. Chromatogr. 1991, 540, 169-175.

Takeuchi, T., Horikawa, R., Tanimura, T., Kabasawa, Y. Resolution of DL-Valine by Countercurrent Solvent Extraction with Continuous Sample Feeding, Sep. Sci. Technol. 1990, 25, 941-951.

Yamazaki, S., Takeuchi, T., Tanimura, T. Direct Enantiomeric Separation of Norephedrine and Its Analogues by High-Performance Liquid Chromatography, J. Liq. Chromatogr. 1989, 12, 2239-2248.

Takeuchi, T., Horikawa, R., Tanimura, T. Enantioselective Solvent Extraction of Neutral DL-Amino Acids in Two-Phase Systems Containing N-n-Alkyl-L-Proline Derivatives and Copper(II) Ions, Anal. Chem. 1984, 56, 1152-1155.

Takeuchi, T., Horikawa, R., Tanimura, T. Complete Resolution of DL-Isoleucine by Droplet Countercurrent Chromatography, J. Chromatogr. 1984, 284, 285-288.

研究業績のトップに戻る

著書・総説

Takeuchi, T. Protein-sensing using organic/inorganic hybrid materials prepared by liquid-phase deposition-based molecular imprinting, In: Lee, S-W, Kunitake T. Handbook of Molecular Imprinting 2013, pp. 487-497, Pan Stanford Publishing Pte Led.

Takeuchi, T., Hishiya, T. Molecular Imprinting of Proteins Emerging as a Tool for Protein Recognition, Org. Biomol. Chem., 2008, 6, 2459?2467

M. Komiyama, T. Takeuchi, T. Mukawa, H. Asanuma, "Molecular Imprinting", WILEY-VCH, Weinheim, 2002.

Matsui, J., Takeuchi, T. Techniques for the In Situ Preparation of Imprinted Polymers, In Molecularly Imprinted Polymers: Man made Mimics of Antibodies and Their Application in Analytical Chemistry, Sellergren, B. Ed., Elsevier, pp. 325-340, 2001.

Takeuchi, T., Shionoya, M. Synthetic Receptors Prepared by Organized Assembly of Organic Molecules, IEEE EMB magazine, 2002, 21 (6), 144-150.

Takeuchi, T., Haginaka, J. Separation and Sensing Based on Molecular Recognition Using Molecularly Imprinted Polymers, J. Chromatogr. B 1999, 728, 1-20.

Takeuchi, T., Matsui, J. Recognition of Drugs and Herbicides: Strategy in Selection of Functional Monomers in Non-covalent Molecular Imprinting, in Molecular and Ionic Recognition with Imprinted Polymers, ACS Symp. Series 703, ACS Publications, Washington, DC, 1998, pp. 119-134.

Takeuchi, T. Matsui, J. Molecular Imprinting: an Approach to Tailor-made Synthetic Polymers with Biomimetic Functions, Acta Polym. 1996, 47, 471-484.

Karube, I., Takeuchi, T. and Barnes, D. J. Biotechnological Reduction of CO2 Emissions, Adv. Biochem. Eng. Biotechnol. 1992, 46, 63-79.

砂山博文, 竹内俊文「分子インプリント高分子の蛍光センシングの応用」高分子2012,61,406-409.

竹内俊文 「モレキュラーインプリンティング」ラジカル重合ハンドブック,蒲池幹治,遠藤剛,岡本佳男,福田猛 監修 2010,第4編,第2章,第2節(NTS)

竹内俊文, 大谷亨 「モレキュラーインプリント法による人工酵素」酵素利用技術大系, 小宮山眞監修, 2010, 第4編, 第6節(NTS).

竹内俊文, 大谷亨 「分子インプリンティングのバイオ応用」ナノ空間材料の創製と応用, 有賀克彦編 2009, 第5章, 第5節(フロンティア出版)

大谷 亨, 竹内俊文「モレキュラーインプリンティング −最近の展開−」高分子 2008, 57, 903-911.

竹内俊文「モレキュラーインプリンティングによるタンパク質認識材料の創製」Electrochemistry(電気化学および工業物理化学) 2007, 75. 986-991.

竹内俊文, 菱谷隆行, 松井 淳「分子インプリント材料を分子素子として用いたセンサー」バイオセンサーの先端科学技術と応用, 民谷栄一監修 2007, 8章 (シーエムシー出版).

竹内俊文, 新森英之「分子インプリントポリマーを用いたセンシング」バイオセンサ・ケミカルセンサ事典, 軽部征夫編 2007, 2章 第5節-2 (テクノシステム).

竹内俊文 「高分子プローブとしてのモレキュラーインプリントポリマー」, 高分子 2003, 52, 458-461.

竹内俊文 「モレキュラーインプリンティング」 生命科学のニューセントラルドグマ,杉本直巳編 2002,19章.

竹内俊文,「モレキュラーインプリンティングによる分子認識材料の創製」,バイオサイエンスとインダストリー 2000,58,17-22.

竹内俊文 「モレキュラーインプリンティング」 ラジカル重合ハンドブック, 蒲池幹治・遠藤剛監修(エヌ・ティー・エス), pp. 645-654, 1999.

竹内俊文,久保裕之 モレキュラーインプリントアフィニティ材料,ぶんせき,1998, No. 11, 840-846.

松井、竹内 「モレキュラーインプリンティング-人工アフィニティ材料の創製-」 化学と工業 1997, 50, 597-598.

竹内 「レセプターを用いる薬物の定量-天然及び人工レセプターを利用して-」臨床化学 1997, 26, 1-6.

竹内 「モレキュラーインプリンティング-分子情報を人工高分子内に刷り込む-」蛋白質核酸酵素 1997,42, 1320-1326.

釘宮章光、松井淳、竹内俊文「モレキュラーインプリンティングによるテーラーメイド分子認識ポリマーの合成」酵素工学ニュース 1996 (35), 6-9.

松井淳, 竹内俊文「テーラーメイド分子認識ポリマー」化学と工業 1995, 48, 1259-1261.

竹内俊文, 村上裕二「酵素センサー」膜学実験シリーズ第2巻生体機能類似膜編(日本膜学会編)pp 180-190, 1994.

竹内俊文「細胞、オルガネラ、組織を用いるセンサー」膜学実験シリーズ第2巻生体機能類似膜編(日本膜学会編)pp 209-214, 1994.

竹内俊文, 軽部征夫「非放射性レセプターアッセイの開発」化学 1993, 48, 578-579.

軽部征夫, 竹内俊文「マイクロマシン技術を応用した集積型フローインジェクション分析システム」化学 1992, 47, 434-435.

竹内俊文「IMBC '91 国際会議」酵素工学ニュース 1992, 27, 36.

竹内俊文「ハワイのハイテク事情」化学と工業 1991, 44, 688-689.

研究業績のトップに戻る

 

 研究業績のトップに戻る


 

学外資金の導入状況

 


社会との連帯(広島バイオアカデミー)

 我々は、大学における社会との連帯の重要性を鑑み、広島市立大学在籍の当初(開学の年,平成6年)より広島先端科学技術セミナーを開催し、学外の研究者・技術者・一般の方々と交流する事に努めた。また、平成7年度は、広島市立大学特定研究(公募型学内研究費)の助成により、少人数制バイオ研修コースを設け、学外者と交流した。平成8年以降、この研修コースは、残念ながら予算の関係で打ち切ったが、以後竹内グループで主導的に行うこのような参加費無料の交流の場を「広島バイオアカデミー」と名付け、出来る限り学外者との交流を継続して行うことにした。

 

 広島先端科学技術セミナー

第5回(平成10年度 詳細

1. 「高分子ミセルー薬物標的治療のためのインテリジェントマテリアルーその魅力と可能性」 東京大学大学院工学系研究科教授 片岡 一則 氏 

2. 「遺伝子診断薬としてのDNAコンジュゲート」 九州大学大学院工学研究科教授 前田 瑞夫 氏

第4回(平成9年度 詳細

1. 「次世代のバイオテクノロジー:アンチセンス法、コンビナトリアルケミストリー、インビトロセレクション」 甲南大学理学部教授 杉本 直己 氏 

2. 「DNAチップと高機能性マイクロデバイス」 徳島大学薬学部教授 馬場 嘉信 氏

第3回(平成8年度 詳細

1. 「超生物機能に挑む」 東京工業大学生命理工学部教授 相澤益男氏

2. 「ハイテク技術と地方産業振興」 東京大学先端科学技術研究センター教授 児玉文雄氏

第2回(平成7年度 詳細

1.「生物の毒を追って」 (財)サントリー生物有機化学研究所所長 中嶋輝躬氏

2.「マリンバイオテクノロジーの展望」 東京農工大学工学部教授 松永 是氏

3.「バイオテクノロジーを利用した新しい環境計測」 北陸先端科学技術大学院大学教授 民谷栄一氏

4.「21世紀に向けた新しい農薬」 日産化学(株)企画研究部長 上田国博氏

第1回(平成6年度 詳細

1.「生体情報とマルチメディア-」 東京大学先端科学技術研究センター教授 軽部征夫氏

 研修コース(平成7年度 詳細

バイオエレクトロニクスコース(平成7年11月〜12月 詳細

「食品の品質管理用バイオセンサー」 (株)ニチレイ味覚評価室 林 研司氏(11月10日)

「メディエーター型バイオセンサー」 北陸先端科学技術大学院大学助教授 横山憲二氏(11月17日)

「体調モニタリング用非侵襲バイオセンサー」 (株)デンソー基礎研究所 三林浩二氏(12月1日)

「実用化されたバイオセンサー」 電気化学計器(株)浅野泰一氏(12月8日)

ヘルスバイオサイエンスコース(平成8年1月〜2月 詳細

「機能性食品と特定保険用食品」 東京大学農学部教授 荒井綜一氏(1月8日)

「高圧バイオサイエンスと食品科学工学」 京都大学農学部教授 林 力丸氏(1月19日)

「核酸的機能性分子の開発とその医薬品・農薬への応用」 甲南大学理学部教授 杉本直己氏(1月22日)

「バイオテクノロジーを用いたオリゴ糖の生産とその応用」 塩水港精糖(株) 橋本 仁氏(2月15日)

「From Academic Research to Production」 Otto Doblhoff-Dier, University of Agriculture, Vienna, Austria(2月21日)